Open Dataset #XSZGHQLG
SEC-SAXS-MALS for mAb4 ( SinoBiological: 40143-R004) + mAb1 ( SinoBiological: 40143-MM08 ) + NPNTD
Abstract: We conducted a structural characterization of mAbs against the N-terminal domain of nucleocapsid protein (NPNTD) from SARS-CoV-2 using small-angle X-ray scattering (SAXS). Our solution-based results distinguished the mAbs’ flexibility and how this flexibility impacts the assembly of multiple mAbs on an antigen. By pairing two mAbs that bind different epitopes on the NPNTD, we show that flexible mAbs form a closed sandwich-like complex. With rigid mAbs, a juxtaposition of the Fabs is prevented, enforcing a linear arrangement of the mAb pair, which facilitates further mAb polymerization.
Experimental Description: For SEC-MALS-SAXS experiments, 60 uL of a sample containing mAb1 ~1.5 mg/mL + mAb4 ~1.5 mg/ml + NPNTD in 1:5 molar ratio was prepared in PBS pH 7.4 buffer. SEC-MALS-SAXS was collected at the SIBYLS beamline (BL 12.3.1) at the Advanced Light Source (ALS) at Lawrence Berkeley National Laboratory (LBNL) in Berkeley, California.
Sample names and types:
- mAb1 | SinoBiological 40143-MM05 | Protein
- mAb4 | SinoBiological 40143-R004 | Protein
- NPNTD | N-terminus of the nucleocapsid protein from SARS-CoV-2 | Protein
File description: *.sec > unsubtracted SAXS curves for 600 two-second exposures across the SEC elution profile ready for SCATTER analysis *.dat > final merged SAXS profile for main SEC peak *pdf > MALS results
Data collection technique: SEC-SAXS
Submitted By: Michal Hammel | [email protected]
Project Leader: Michal Hammel | [email protected]
Additional Authors: https://www.biorxiv.org/content/10.1101/2021.01.13.426597v1
Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline
Country: United States of America
Data Download Terms:
Complete Set of SAXS Data Files:
The complete SAXS dataset is downloadable as a zip file.
- mAb1_mAb4_NPNTD.sec.zip Download
Supplemental Data and Supporting Materials (total 1):
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- Covid_anti2[070920_Covid].pdf Download
Sample SAXS Data Files (total 1):
These are individual SAXS data files that are included in the complete dataset, but are available separately for quick download as examples of what is contained in the zip file.
- mAb1_mAb4_NPNTD.dat Download
Open SAXS data analysis sites in a new tab using these links:SAXS Similarity SAXS FrameSlice