Instructions

Registration

Registration and Sign-in are not required to view and download available data and its corresponding metadata
Registration and Sign-in are required to upload and publish data

Viewing and Downloading Available Data

Navigate to the Dashboard to see the lastest releases, or the OpenData page to see all the available data. You may also search for specific data using the search input field in the navigation bar
Click the View link for the dataset that you would like more information on and/or download
To download the complete dataset, click the Download link or main button under Complete Set of SAS Data Files
There may also be supporting files containing additional information about the experiment, or files such as Multi-angle Light Scattering (MALS), X-ray coordinate files in Protein Data Bank format, etc. Simply click the corresponding Download link for each file

Depositing Data

Register

Read the Terms of Service and Privacy Policy pages
Go to the Registration page and register
Complete your registration by adding in information about your Institute, Project Leader after gaining permission (see Terms of Service), and your Country

Navigate to the Deposit New Dataset Page

Once permissions are granted by our staff, each User is allowed to upload up to 10 datasets per day
While the data is not curated, the datasets will be checked prior to publishing for convention conformity
Sign-in to the website
Click the Deposit link in the top navigation bar

Contributor Information

Required: Select your Institution (University, Company, .etc). If your institute is not listed, again, use the Add new institution to pulldown link
Required: Choose a Project Leader (Principal Investigator, Lead Scientist, .etc) from the pulldown menu. If you are the project leader, and have not done so already, click the Add new project leader to pulldown link and add yourself. You may need to refresh the Deposit New Dataset page to then find yourself in the pulldown
Required: Select your Country

Experimental Description

Required: Add a short Title for the dataset
Required: Select the Data collection technique
Required: Provide an Abstract. This should be a description of the objective or why you did your experiment, along with expectations and results, .etc

Human superoxide dismutase (SOD) catalyzes the conversion of the free radical superoxide to molecular oxygen and water. Some mutations within the SOD1 gene gives rise to mutant proteins that are implicated in amyotrophic lateral sclerosis. When these mutants are isolated and incubated at 37 degrees C, we determined that they then form filaments and aggregate. We tested whether increasing amounts of copper could stabilize the SOD mutant D90A using SAXS. We found that...
Required: Provide an Experimental description. This should be how you did your experiment, ie. the methods:

Recombinant human superoxide dismutase 1 (SOD1) with the mutation D90A was expressed in E. coli and purified with a final buffer of 100 mM Tris-Cl, 200 mM NaCl, and 1 mM TCEP. The protein was then incubated in varying amounts of CuSO4 and/or ZnCl2 and heated to 37 degrees C for 15 min. Samples were subjected to X-rays at wavelength 1.000 Å at Beamline 12.3.1 at the Advanced Light Source...
Required: Provide a File Description for your dataset. This should be a roadmap so that others can determine what the experimental conditions are for each file such that another user can identify what they represent:

Each file has been processed such that a "blank" scattering curve representing the buffer plus the appropriate amount of CuSO4 or ZnCl2 has been subtracted. No further processing has been done. Files sod_cu_001.dat to sod_cu_050.dat represent the SOD D90A protein incubated in 1 to 50 mM CuSO4 in 1 mM increments. Files sod_zn_051.dat to sod_zn_100.dat represent protein incubated in 1 to 50 mM CuSO4 in 1 mM increments. Files sod_cuzn_101.dat to sod_cuzn_150.dat represent protein incubated in 1 to 50 mM CuSO4 and ZnCl2 in 1 mM increments. An associated X-ray crystal structure coordinate file in .pdb format of SOD D90A is also included.

Sample information

Required: Select the sample type (Protein, RNA, Detergent etc.) from the pulldown
Required: Fill in the macromolecule's abbreviated name, e.g. SOD1 | 5S rRNA | Sucrose Monocaprate
Required: Fill in the macromolecule's full name, e.g. Cu,Zn superoxide dismutase | 5S ribosomal RNA | β-D-Fructofuranosyl Monodecanoate
Optional (Required for Proteins/Nucleic Acids): Chemical Formula or Sequence. Please use the formula only, do not add in headers from files such as fasta, e.g. MSAAKIHHHHHH | ATGATCTTATAA | C22H40O12
Optional: Fill in the Source Organism and the NCBI Taxonomy ID, e.g. Homo sapiens & 9606, respectively
Optional: Fill in the Expression Organism and the NCBI Taxonomy ID e.g. Escherichia coli & 562 , respectively
Optional: For proteins, fill in the Uniprot Identifier, e.g. B6CHW7 for Alvinella pompejana SOD

Data Files

Required for large datasets:

zip file

only

Each file within the compressed zip file should contain plain text, with three columns representing the scattering angles, intensities and errors
Each file within the compressed zip file should have the extension .dat
Do not add images to your zip file, or other supporting material
The file must have the extension .zip
There is currently a memory limit of 1 KB to 20 MB for the zip file
Only one zip file is allowed per dataset

Required for small datasets:

These files should also be included in your main dataset
Each file should contain plain text, with three columns representing the scattering angles, intensities and errors
The files should have the extension .dat
The memory limit for each file is 1 to 300 KB
There is a limit of up to 100 files that may be submitted this way

Optional Information

Supplemental Data and Supporting Materials

Optional:

Support files with further descriptions of the project or additional details of the file contents in the zip file or individual SAS data should be in either the portable document format or plain text with the .pdf or .txt extensions, respectively
Modelled or X-ray crystal structure atomic coordinates should have the .pdb or .cif extension
Tables or spreadsheets should have the .csv format and extension
Do not upload journal articles
The memory limit for each file is 1 to 10 MB
There is a combined limit of up to 6 support files

Journal Information

Optional:

Enter the full url such as "https://doi.org/10.1038/nature15646"
Or copy and paste the identifier shown on sites such as NCBI PubMed, e.g. "10.1038/nature15646"
Do not use urls that point directly to journal articles

Experimental Metadata

Optional:

SAS Source: Synchrotrons, Nuclear reactors and Spallation neutron sources, Home sources, etc.
The particular beamline or instrument
Data collection wavelength in angstroms
Sample to detector distance in meters

Collaborators

Optional:

Click the "Add Collaborator" link to add the first person
Fill in the first name, middle initial, last name of the person
Add their email address
Add their affiliation/institute
Click "Add Collaborator" link again to add the next collaborator

Editing or Deleting Your Data

Once you have submitted your data, you can find it later using the MyDatasets link in the top navigation bar
After you have identified your dataset, you may click either the Show, Edit, or Delete links to perform the corresponding action
Warning - you will not be able to recover your data if you choose to delete it

Releasing Your Data to the Public

Once you are ready to release your data to the public, go to the Edit page for the particular dataset, as shown above, and click the Publish Dataset radio button, and click the Submit Status button.
Your dataset will be reviewed for adhering to site policy and conformity, we will however not validate your data. Following review, your data will then be published.
Warning - Currently, once sent to pending status, you will not be able to revert the action
Warning - If you delete your account, your data will also be deleted