Open Dataset XSWB6KOE

Cellobiose Dehydrogenase - wild type and its variants (pH range)


The interdomain electron transfer between the catalytic flavodehydrogenase domain and the electron transferring cytochrome domain of cellobiose dehydrogenase plays an essential role in biocatalysis, biosensors, and biofuel cells as well as for its natural function as an auxiliary enzyme of lytic polysaccharide monooxygenase. We revealed the role of two amino acids (M309 and R698) located in the domain interface which are hypothesized to limit interdomain electron transfer. CDH from Myriococcum thermophilum was used for site-specific mutagenesis. The wild-type and all variants (M309A, R698S and M309A/R698S) were analyzed kinetically by stopped-flow studies and structurally by using small-angle X-ray scattering. We showed an increase of CDH flexibility at higher pH, indicating alterations of domain mobility.

Experimental description

The sample used for the experiments represents MtCDH IIA: gene cdh, UniProt A9XK88, the CYT domain belongs to CAZy family AA8, and the DH domain to CAZy family AA3_1. SEC-SAXS data were collected at the SIBYLS beamline 12.3.1 at Advanced Light Source (LBNL Berkeley, California, USA) at 1.27 Å wavelength with a Pilatus 2M detector 2.1 m sample-to-detector distance, corresponding to a q range from 0.01 to 0.4 Å-1. The scattering vector is defined as q = 4π sinθ/λ, where 2θ is the scattering angle, and λ is the X-ray wavelength. For the pH-profile measurements a 50 mM phosphate-citrate buffer system was used. A size-exclusion chromatography was directly coupled with a SAXS flow cell and a multi-angle light scattering (SEC-SAXS-MALS). For analyzing the data and computing the pair distance distribution function the program SCÅTTER ( was used. The SAXS frames recorded before the protein elution was used for the background subtraction.

File description

MtCDH.. are the files for the wild-type M309A..are the files for the M309A variant R698S..are the files for the R698S variant M309AR698S..are the files for the double mutant variant





Data collection technique


Journal DOI


Advanced Light Source




1.27 Å

Sample to Detector Distance

2.1 m

Submitting Author

Bettina Motycka

University of Natural Resources and Life Sciences, Vienna (BOKU)


[email protected]


Project Leader

Michal Hammel

[email protected]

Data Download Terms:

The data available for download is free to use, however by clicking a download link, it signifies that you agree to our Terms of Service and Privacy Policy.

Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.

Individual SAS Data Files (total 20)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.

Supplemental Data and Supporting Materials (total 0)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

Open SAXS data analysis sites in a new tab using these links

SAXS Similarity SAXS FrameSlice


  • Macromolecule 1: MtCDH
    • Sample Full Name: Cellobiose dehydrogenase
    • Sample Type: Protein
    • Source Organism: Myriococcum thermophilum
    • Source Organism NCBI Taxonomy ID: 2588717
    • Expression System: Pichia pastoris X-33
    • Expression NCBI Taxonomy ID: 4922
    • Uniprot ID: A9XK88
    • Sequence or Chemical Formula: