Open Dataset XSW9GJYN
SEC-SAXS-MALS for mAb2 ( SinoBiological: 40143-MM08) against the N-terminal domain of nucleocapsid protein from SARS-CoV-2
We conducted a structural characterization of mAbs against the N-terminal domain of nucleocapsid protein (NPNTD) from SARS-CoV-2 using small-angle X-ray scattering (SAXS). Our solution-based results distinguished the mAbs’ flexibility and how this flexibility impacts the assembly of multiple mAbs on an antigen. By pairing two mAbs that bind different epitopes on the NPNTD, we show that flexible mAbs form a closed sandwich-like complex. With rigid mAbs, a juxtaposition of the Fabs is prevented, enforcing a linear arrangement of the mAb pair, which facilitates further mAb polymerization.Experimental description
For SEC-MALS-SAXS experiments, 60 uL of a sample containing mAb2 ~1.5 mg/mL was prepared in PBS pH 7.4 buffer. SEC-MALS-SAXS was collected at the SIBYLS beamline (BL 12.3.1) at the Advanced Light Source (ALS) at Lawrence Berkeley National Laboratory (LBNL) in Berkeley, California.File description
*cbf.dat > unsubtracted SAXS curves for 600 two-second exposures across the SEC elution profile. *.dat > final merged SAXS profile for main SEC peak *.pdb > two multistate atomic model *.pdf > SEC-MALS results
2021-05-21Data collection technique
Sample to Detector Distance
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Data Download Terms:
Complete Set of SAS Data Files
The complete SAS dataset is downloadable as a zip file.
- mAb2.zip Download
Individual SAS Data Files (total 1)
These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.
- ant2_new.dat Download
Supplemental Data and Supporting Materials (total 3)
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- Covid_anti2alonne[111320_Seq1].pdf Download
- 1igt45_2_176000.pdb Download
- 1igt65_2_180500.pdb Download
Open SAXS data analysis sites in a new tab using these linksSAXS Similarity SAXS FrameSlice