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Open Dataset XSVC6W5N

SEC-SAXS data on Non Structural Proteins 7, 8 & 12 from SARS- CoV2

Abstract:

The three proteins, Non Structural Proteins 7 8 & 12 or NSP 7, NSP 8 and NSP 12 are components of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co-expressed in E. Coli and co- purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

Experimental description:

The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5.

File description:

Each file name is numerically iterated so that NSP7-8-12_B3_3_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording.

Data collection technique:

SEC-SAXS

Wavelength

Sample to Detector Distance

Created: 2021-03-08

Updated: 2021-05-21

Submitting Author:

Greg Hura

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

[email protected]

Collaborators:

Michal Hammel, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim

Project Leader:

Greg Hura

[email protected]


Data Download Terms:

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Complete Set of SAXS Data Files:

The complete SAXS dataset is downloadable as a zip file.

Sample SAXS Data Files (total 0):

These are individual SAXS data files that are included in the complete dataset, but are available separately for quick download as examples of what is contained in the zip file.

Supplemental Data and Supporting Materials (total 1):

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

  • Nsp12 + Nsp7_Nsp8 B3[052220_COVID_5].pdf Download

Open SAXS data analysis sites in a new tab using these links:

SAXS Similarity SAXS FrameSlice

Samples:

  • Macromolecule 1: NSP7
    • Sample Full Name: Non-structural Protein 7
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula:
  • Macromolecule 2: NSP8
    • Sample Full Name: Non-structural Protein 8
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula:
  • Macromolecule 3: NSP12
    • Sample Full Name: Non-structural Protein 12
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula: