Open Dataset #XSVC6W5N

SEC-SAXS data on Non Structural Proteins 7, 8 & 12 from SARS- CoV2

Abstract: The three proteins, Non Structural Proteins 7 8 & 12 or NSP 7, NSP 8 and NSP 12 are components of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co-expressed in E. Coli and co- purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

Experimental Description: The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5.

Status: Published

Sample names and types:

  • NSP7 | Non-structural Protein 7 | Protein
  • NSP8 | Non-structural Protein 8 | Protein
  • NSP12 | Non-structural Protein 12 | Protein

File description: Each file name is numerically iterated so that NSP7-8-12_B3_3_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording.

Data collection technique: SEC-SAXS

Submitted By: Greg Hura | [email protected]

Project Leader: Greg Hura | [email protected]

Additional Authors: Michal Hammel, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim

Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline

Country: United States of America

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Complete Set of SAXS Data Files:

The complete SAXS dataset is downloadable as a zip file.

Supplemental Data and Supporting Materials (total 1):

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

  • Nsp12 + Nsp7_Nsp8 B3[052220_COVID_5].pdf Download

Sample SAXS Data Files (total 0):

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SAXS Similarity SAXS FrameSlice