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Open Dataset XSVC6W5N

SEC-SAXS data on Non Structural Proteins 7, 8 & 12 from SARS- CoV2

Abstract

The three proteins, Non Structural Proteins 7 8 & 12 or NSP 7, NSP 8 and NSP 12 are components of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co-expressed in E. Coli and co- purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

Experimental description

The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5.

File description

Each file name is numerically iterated so that NSP7-8-12_B3_3_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording.

Created

2021-03-08

Published

2021-05-21

Data collection technique

SEC-SAXS

Journal DOI

Source

Beamline

Wavelength

Sample to Detector Distance

Submitting Author

G. Hura

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

Collaborators

M. Hammel, D. Rosenberg, M. Wilamowski, A. Joachimiak, Y. Kim

Project Leader

G. Hura


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Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.

Individual SAS Data Files (Total: 0)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.

Supplemental Data and Supporting Materials (Total: 1)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

  • Nsp12 + Nsp7_Nsp8 B3[052220_COVID_5].pdf Download

Open SAXS data analysis sites in a new tab using these links

SAXS Similarity SAXS FrameSlice

Samples:

  • Macromolecule 1: NSP7
    • Sample Full Name: Non-structural Protein 7
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula:
  • Macromolecule 2: NSP8
    • Sample Full Name: Non-structural Protein 8
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula:
  • Macromolecule 3: NSP12
    • Sample Full Name: Non-structural Protein 12
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula: