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Open Dataset XSVC6W5N

SEC-SAXS data on Non Structural Proteins 7, 8 & 12 from SARS- CoV2

Abstract

The three proteins, Non Structural Proteins 7 8 & 12 or NSP 7, NSP 8 and NSP 12 are components of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co-expressed in E. Coli and co- purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

Experimental description

The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5.

File description

Each file name is numerically iterated so that NSP7-8-12_B3_3_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording.

Created

2021-03-08

Published

2021-05-21

Data collection technique

SEC-SAXS

Journal DOI

Source

Beamline

Wavelength

Sample to Detector Distance

Submitting Author

Greg Hura

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

[email protected]

Collaborators

Michal Hammel, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim

Project Leader

Greg Hura

[email protected]


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Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.

Individual SAS Data Files (Total: 0)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.

Supplemental Data and Supporting Materials (Total: 1)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

  • Nsp12 + Nsp7_Nsp8 B3[052220_COVID_5].pdf Download

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SAXS Similarity SAXS FrameSlice

Samples:

  • Macromolecule 1: NSP7
    • Sample Full Name: Non-structural Protein 7
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula:
  • Macromolecule 2: NSP8
    • Sample Full Name: Non-structural Protein 8
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula:
  • Macromolecule 3: NSP12
    • Sample Full Name: Non-structural Protein 12
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula: