Open Dataset XSUTRZQL

Apoptosis-inducing factor (AIF) allostery mutants and their impact on NADH-driven dimer formation

Abstract: Oxidoreductase Apoptosis-inducing factor (AIF) supports critical mitochondrial activities, including OXPHOS biogenesis and cristae remodeling, and participates in the PARP-1-dependent cell death pathway parthanatos. These functions are seemingly regulated by allosteric monomer-dimer switching driven by binding and reduction by NADH. Using small-angle X-ray scattering (SAXS), we characterized a panel of monomer and dimer-permissive mutants to define molecular pathways linking AIF’s NADH active site to allosteric switching regions. These results were combined with X-ray crystallography and molecular dynamics and reported in “Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor". Brosey CA, Ho C, Long WZ, Singh S, Burnett K, Hura GL, Nix JC, Bowman GR, Ellenberger T, Tainer JA. Structure, 6:2067-2079 (2016). DOI: 10.1016/j.str.2016.09.012.

Experimental Description: N-terminally truncated AIF (78-613) and point mutants were expressed in bacteria and purified by nickel affinity and gel filtration chromatography (expression tags were removed post-nickel purification). Purified proteins were concentrated and exchanged into SAXS buffer by size-exclusion chromatography (Superdex 200 10/300) to ensure monodisperse protein. SAXS samples were prepared at 3-4 mg/mL with direct addition of NADH ligand to protein and SEC-matched buffers. HT-SAXS data were collected by mail-in service at ALS beamline 12.3.1 (SIBYLS) at Lawrence Berkeley National Laboratory. The dataset available here contains wild-type AIF (78-613) and point mutants collected at 3-4 mg/mL with and without 10-fold molar excess NADH. Additional I(q) curves include the AIF-ΔC-loop mutant (C-loop dataset), which replaces AIF’s allosteric C-terminal loop (residues 511-558) with a GGGSSS insert, and a second truncation mutant (AIF121 datasets, residues 121-613) that completely removes AIF’s flexible N-terminus.

File description: File names are descriptive. Wild-type AIF is designated as WT. N-terminal truncations are denoted as AIF78 (residues 78-613) or AIF121 (residues 121-613). Point mutations or the C-loop deletion are as indicated in the AIF78 background. The presence of NADH is also noted.

Created: 2022-07-14

Updated: 2022-07-15

Submitting Author:

Chris Brosey | [email protected]

Data collection technique:


Project Leader:

John Tainer | [email protected]

Additional Authors:


University of Texas MD Anderson Cancer Center


United States of America

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Complete Set of SAXS Data Files:

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Sample SAXS Data Files (total 1):

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Supplemental Data and Supporting Materials (total 2):

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  • Macromolecule 1: AIF
    • Sample Full Name: Apoptosis-Inducing Factor
    • Sample Type: Protein
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