Open Dataset #XSTKZWCR
SEC-SAXS-MALS for NSP7/NSP8 + dsRNA from SARS-CoV2
Abstract: Mixing of NSP7/NSP8 with dsRNA leads to NSP8/dsRNA complexation
Experimental Description: The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non-Structural Proteins 7, and 8 are a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co- expressed in E. Coli and co-purified, sent to SIBYLS for collection. NSP7/8 was mixed with an equimolar concentration of dsRNA and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis
Sample names and types:
- NSP7 | Non-structural Protein 7 | Protein
- NSP8 | Non-structural Protein 8 | Protein
- dsRNA | Double-stranded RNA | RNA
File description: Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP78_dsRNAdat file for an SEC peak region is included.
Data collection technique: SEC-SAXS
Submitted By: Michal Hammel | [email protected]
Project Leader: Michal Hammel | [email protected]
Additional Authors: Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim
Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline
Country: United States of America
Data Download Terms:
Complete Set of SAXS Data Files:
The complete SAXS dataset is downloadable as a zip file.
- NSP8_7_dsRNA.zip Download
Supplemental Data and Supporting Materials (total 2):
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
Sample SAXS Data Files (total 1):
These are individual SAXS data files that are included in the complete dataset, but are available separately for quick download as examples of what is contained in the zip file.
- nsp78rna.dat Download
Open SAXS data analysis sites in a new tab using these links:SAXS Similarity SAXS FrameSlice