Open Dataset #XSTKZWCR


SEC-SAXS-MALS for NSP7/NSP8 + dsRNA from SARS-CoV2

Abstract: Mixing of NSP7/NSP8 with dsRNA leads to NSP8/dsRNA complexation

Experimental Description: The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non-Structural Proteins 7, and 8 are a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co- expressed in E. Coli and co-purified, sent to SIBYLS for collection. NSP7/8 was mixed with an equimolar concentration of dsRNA and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis


Status: Published

Sample names and types:

  • NSP7 | Non-structural Protein 7 | Protein
  • NSP8 | Non-structural Protein 8 | Protein
  • dsRNA | Double-stranded RNA | RNA

File description: Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP78_dsRNAdat file for an SEC peak region is included.

Data collection technique: SEC-SAXS

Submitted By: Michal Hammel | [email protected]

Project Leader: Michal Hammel | [email protected]

Additional Authors: Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim

Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline

Country: United States of America

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Complete Set of SAXS Data Files:

The complete SAXS dataset is downloadable as a zip file.


Supplemental Data and Supporting Materials (total 2):

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Sample SAXS Data Files (total 1):

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