Open Dataset #XSPRDTAA

SEC-SAXS-MALS for mAb4 ( SinoBiological: 40143-R004) against the N-terminal domain of nucleocapsid protein from SARS-CoV-2

Abstract: We conducted a structural characterization of mAbs against the N-terminal domain of nucleocapsid protein (NPNTD) from SARS-CoV-2 using small-angle X-ray scattering (SAXS). Our solution-based results distinguished the mAbs’ flexibility and how this flexibility impacts the assembly of multiple mAbs on an antigen. By pairing two mAbs that bind different epitopes on the NPNTD, we show that flexible mAbs form a closed sandwich-like complex. With rigid mAbs, a juxtaposition of the Fabs is prevented, enforcing a linear arrangement of the mAb pair, which facilitates further mAb polymerization.

Experimental Description: For SEC-MALS-SAXS experiments, 60 uL of a sample containing mAb4 ~1.5 mg/mL was prepared in PBS pH 7.4 buffer. SEC-MALS-SAXS was collected at the SIBYLS beamline (BL 12.3.1) at the Advanced Light Source (ALS) at Lawrence Berkeley National Laboratory (LBNL) in Berkeley, California

Status: Published

Sample name and type:

  • mAb4 | mAb4 ( SinoBiological: 40143-R00) | Protein

File description: *cbf.dat > unsubtracted SAXS curves for 600 two-second exposures across the SEC elution profile. *.dat > final merged SAXS profile for main SEC peak *.pdb > two multistate atomic model *.pdf > SEC-MALS results

Data collection technique: SEC-SAXS

Submitted By: Michal Hammel | [email protected]

Project Leader: Michal Hammel | [email protected]

Additional Authors:

Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline

Country: United States of America

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Complete Set of SAXS Data Files:

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Supplemental Data and Supporting Materials (total 3):

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Sample SAXS Data Files (total 1):

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SAXS Similarity SAXS FrameSlice