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Open Dataset XSPDWNXI

Physicochemical and structural insights into lyophilized mRNA-LNP from lyoprotectant and buffer screenings

Abstract

The delivery of messenger RNA (mRNA) via lipid nanoparticles (LNPs) is rapidly gaining popularity as an effective method for developing therapeutics and vaccines. For example, COVID-19 vaccines used this approach. Employing small-angle X-ray scattering (SAXS), we examined the efficacy of a variety of compounds including amino acides, oligosaccharides, and polymers in different buffers for their ability to stabilize mRNA-LNPs during lyophilization. We did these experiments in an effort to circumvent the need to store and transport mRNA-LNPs under extremely cold temperatures. In conjuction with cryogenic transmission electron microscopy data and cellular assays, these SAXS data suggest some formulations provide enhanced protection during lyophilization, such as polyvinylpyrrolidone in tris or acetate buffers. For more information, see the corresponding journal article linked below.

Experimental description

SAXS data were collected at the SIBYLS beamline 12.3.1 at the Advanced Light Source. Experiments were performed with an X-ray wavelength of 1.216 Å, a sample-to-detector distance 2.07 m, and 10 second exposures with a 0.3 second frame rate to maximize signals. The scattering vector, q, ranged from 0.01 Å−1 to 0.45 Å−1.Data were merged with SAXS FrameSlice, and scattering profiles were superimposed by offsetting the data in BioXTAS RAW to achieve a consistent baseline. The data were analyzed with OriginPro 2022b from OriginLab as reported in our journal article.

File description

All data in the ZIP file contain final merged SAXS scattering profiles from lipid nanoparticles loaded with mRNA (mRNA-LNPs). File names indicate Fresh/Lyophilized, percent of excipient, type of excipient, and buffer.

Created

2024-03-27

Published

2024-12-13

Data collection technique

HT-SAXS

Journal DOI

https://doi.org/10.1016/j.jconrel.2024.07.052

Source

Advanced Light Source

Beamline

SIBYLS BL12.3.1

Wavelength

1.216 Å

Sample to Detector Distance

2.07 m

Submitting Author

Lee Joon Kim

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

[email protected]

Collaborators

Project Leader

Greg Hura

[email protected]


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Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.

  • Fresh_Lyophilized_mRNA_LNP_Screening.zip Download

Individual SAS Data Files (Total: 0)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.

Supplemental Data and Supporting Materials (Total: 0)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

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SAXS Similarity SAXS FrameSlice

Sample:

  • Macromolecule 1: mRNA-LNPs
    • Sample Full Name: Lipid nanoparticles loaded with messenger RNA
    • Sample Type: Lipid Nanoparticle
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Uniprot ID's:
    • Sequence or Chemical Formula: