Open Dataset XSMYUV7S
Gold nanoparticle PARP1 PEITHO assay
We utilize SAXS of gold nanoparticles (AuNP) conjugated with DNA to screen for PARP1 allostery during DNA break binding, using the novel PEITHO compound library. Publication pending.
Experimental descriptionHT SAXS of DNA-conjugated gold nanoparticles + PARP1 or truncated PARP1 protein + small molecules, including PARP1 inhibitors, PEITHO compounds, and NAD+
File descriptionRefer to the Supplemental Data file XSMYUV7S.csv for sample details of each dataset file
2025-04-15
Published2025-04-16
Data collection techniqueHT-SAXS
Journal DOI
Advanced Light Source
BeamlineSIBYLS BL12.3.1
WavelengthSample to Detector Distance
Joshua Del Mundo
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Collaborators
Project LeaderGreg Hura
Data Download Terms:
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Complete Set of SAS Data Files
The complete SAS dataset is downloadable as a zip file.
- XSMYUV7S.zip Download
Individual SAS Data Files (Total: 0)
These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.
Supplemental Data and Supporting Materials (Total: 1)
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- XSMYUV7S.csv Download
Open SAXS data analysis sites in a new tab using these links
SAXS Similarity SAXS FrameSliceSamples:
- Macromolecule 1: dsDNA-AuNP
- Sample Full Name: double stranded DNA gold nanoparticles
- Sample Type: Nanoparticle
- Source Organism:
- Source Organism NCBI Taxonomy ID:
- Expression System:
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- 5’-(AuNP)-TrT-GCC TTC ATA TCT GGA GTG CTT ATT CCT ATC G-3’ 3’-CGG AAG TAT AGA CCT CAC GAA TAA GGA TAG C-TrT-(AuNP)-5’
- Macromolecule 2: SSB-AuNP
- Sample Full Name: single strand break DNA gold nanoparticles
- Sample Type: Nanoparticle
- Source Organism:
- Source Organism NCBI Taxonomy ID:
- Expression System:
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- 5’-(AuNP)-TrT-GCC TTC ATA TCT GGA GTG CTT ATT CCT ATC G-3’ 3’-CGG AAG TAT AGA CCT C*AC GAA TAA GGA TAG C-TrT-(AuNP)-5’
- Macromolecule 3: PARP1
- Sample Full Name: poly(ADP-ribose) polymerase 1
- Sample Type: Protein
- Source Organism: Homo sapiens
- Source Organism NCBI Taxonomy ID:
- Expression System: E coli
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW
- Macromolecule 4: ΔART
- Sample Full Name: ΔART construct of poly(ADP-ribose) polymerase 1
- Sample Type: Protein
- Source Organism: Homo sapiens
- Source Organism NCBI Taxonomy ID:
- Expression System: E coli
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- SDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW
- Macromolecule 5: Zn1-Zn2
- Sample Full Name: Zn1-Zn2 construct of poly(ADP-ribose) polymerase 1
- Sample Type: Protein
- Source Organism: Homo sapiens
- Source Organism NCBI Taxonomy ID:
- Expression System: E coli
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- SDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIF
- Macromolecule 6: Zn1
- Sample Full Name: Zn1 construct of poly(ADP-ribose) polymerase 1
- Sample Type: Protein
- Source Organism: Homo sapiens
- Source Organism NCBI Taxonomy ID:
- Expression System: E coli
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- SDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQ
- Macromolecule 7: Zn2
- Sample Full Name: Zn2 construct of poly(ADP-ribose) polymerase 1
- Sample Type: Protein
- Source Organism: Homo sapiens
- Source Organism NCBI Taxonomy ID:
- Expression System: E coli
- Expression NCBI Taxonomy ID:
- Uniprot ID's:
- Sequence or Chemical Formula:
- QLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIF