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Open Dataset XSHPX9SN


SCOPER validation benchmark (RNA#13) P4P6 (300mM KCl and 5mM Mg++)

Abstract

SCOPER is an efficient tool that can accurately predict the solution state of RNAs and provide an atomistic model of their structure. To validate SCOPER pipeline, we collect experimental SAXS data from a monomeric RNA state, free of contaminants (higher oligomeric state obtain and aggregation). We applied Size Exclusion Chromatography coupled with small angle X-ray scattering (SEC-SAXS).

Experimental description

Data were collected at SIBYLS beamline 12.3.1 at Advanced Light Source with recently reported developed SEC-SAXS-MALS modality (Rosenberg et al. 2022 Methods Enzymol). X-ray wavelength was set at λ=1.127 Å, and the sample to detector distance was 2100 mm, resulting in scattering vectors, q, ranging from 0.01 Å-1 to 0.4 Å-1. A total of 60-95 μL of annealed RNAs with a concentration between 1 and 3 mg/ml were prepared in the SEC running buffer (20mM Tris pH 7.4, 300mM KCl, 5mM MgCl2). The Shodex KW802.5 column was equilibrated with running buffer with a flow rate of 0.65 mL/min. Each sample was injected in an SEC column, and two second X-ray exposures were recorded continuously for 24 min.

File description

p4p6_300mM_5mM.zip: 660 SEC-SAXS frames; best_p4p6_with_mg.dat : SCOPER SAXS fit; original_p4p6.pdb : P4P6 crystal structure; best_p4p6_with_mg.pdb : SCOPER model

Created

2023-03-04

Updated

2023-03-04

Data collection technique

SEC-SAXS

Journal DOI

Source

Beamline

Wavelength

1.127 Å

Sample to Detector Distance

2.1 m


Submitting Author

Michal Hammel

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

[email protected]

Collaborators

Project Leader

Michal Hammel

[email protected]


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Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.


Individual SAS Data Files (total 1)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.


Supplemental Data and Supporting Materials (total 2)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.


Open SAXS data analysis sites in a new tab using these links

SAXS Similarity SAXS FrameSlice


Sample:

  • Macromolecule 1: P4P6
    • Sample Full Name: P4P6 RNA
    • Sample Type: RNA
    • Source Organism: Homo Sapiens
    • Source Organism NCBI Taxonomy ID: 9606
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Uniprot ID:
    • Sequence or Chemical Formula:
    • GAAUUGGGGGAAAGGGGUCAACAGCCGUUCAGUACCAAGUCUCAGGGGAAACUUUGAGAUGGCCUUGCAAAGGGUAUGGUAAUAAGCUGACGGACAUGGUCCUAACCCCCAGCCAAGUCCUAAGUCAACAGAUCUUCUGUUGAUAUGGAUGCAGUUC