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Open Dataset XSG1TQHV


SEC-SAXS-MALS data and models for NSP8 from SARS-CoV2

Abstract:

SEC-SAXS-MALS data on NSP8 from SARS-CoV2

Experimental description:

The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non Structural Protein 8 is a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These protein was co-expressed in E. Coli and co- purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

File description:

File description: Each file name is numerically iterated so that NSP8_3_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP8.dat file for an SEC peak region is included.

Data collection technique:

SEC-SAXS

Wavelength

Sample to Detector Distance


Created: 2021-03-06

Updated: 2021-05-21

Submitting Author:

Michal Hammel

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

[email protected]

Collaborators:

Michal Hammel, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim

Project Leader:

Michal Hammel

[email protected]


Data Download Terms:

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Complete Set of SAXS Data Files:

The complete SAXS dataset is downloadable as a zip file.

  • NSP8_frames+merged_DataFile.zip Download


Sample SAXS Data Files (total 1):

These are individual SAXS data files that are included in the complete dataset, but are available separately for quick download as examples of what is contained in the zip file.


Supplemental Data and Supporting Materials (total 3):

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.


Open SAXS data analysis sites in a new tab using these links:

SAXS Similarity SAXS FrameSlice


Sample:

  • Macromolecule 1: NSP8
    • Sample Full Name: Non-structural Protein 8
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Sequence or Chemical Formula: