Open Dataset XSEVJCYK
Morphologies of Single-Stranded DNA Oligomers Adsorbed on Carbon Nanotube-Based Sensors in Solution using X-ray Scattering Interferometry
Single-walled carbon nanotubes (SWCNTs) with adsorbed nucleic acids are used as sensors in biological experiments. Different single-stranded DNA (ssDNA) sequences alter the specificity of affinities of the ssDNA-SWCNT complexes to different analyte targets. In these experiments, we determined the morphologies of two adsorbed ssDNA oligomers using X-ray scattering interferometry (XSI). Previous to this study, the morphologies of ssDNAs were calculated predominantly via computational models, or where the ssDNA-SWCNTs were dehydrated. We conjugated gold nanoparticles (AuNPs) to (GT)6 or (GT)15 ssDNA oligomers to boost X-ray scattering prior to adsorbing them onto SWCNT surfaces, and performed XSI experiments in solution. The data suggests the (GT)6 oligomers make a series of highly-ordered stacked rings along the the length of the SWCNT, while the (GT)15 oligomers wrap SWCNTs in a less-ordered periodic fashion. Dopamine then elicits a simultaneous axial elongation and radial constriction of the adsorbed ssDNA oligomers on the SWCNT surface. More details are available in the corresponding JACS journal article linked below.
Experimental descriptionAuNPs ranging from 5.9-7.2 nm in diameter were conjugated to the 5’ end of ssDNA oligomers. The AuNP-ssDNAs (6 and 15 nucleotides) were then adsorbed to the surfaces of SWCNTs, forming an AuNP-ssDNA-SWCNT suspension. We then collected XSI in high-throughput mode at the SIBYLS beamline using a Tecan Evo liquid Handling robot. Each sample was irradiated with X-rays at 1.2398 Å for 5 seconds with a 0.1 second frame-rate with a sample to detector distance of 2.07 meters. The data here represent the scattering data with buffer background subtraction.
File descriptionAll X-ray Scattering Interferometry curves for the publication Rosenberg DJ et al. "Mapping the Morphology of DNA on Carbon Nanotube-Based Sensors in Solution using X-ray Scattering Interferometry" J. Am. Chem. Soc. 2024, 146, 386−398. See DOI link below.
2023-05-23
Published2025-02-10
Data collection techniqueHT-SAXS
Journal DOIAdvanced Light Source
BeamlineSIBYLS BL12.3.1
Wavelength1.2398 Å
Sample to Detector Distance2.0 m
Daniel Rosenberg
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Collaborators
- Daniel Rosenberg, [email protected], UCB
- Rebecca Pinals, [email protected], MIT
Greg Hura
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SAXS Similarity SAXS FrameSliceSample:
- Macromolecule 1: ssDNA-AuNP-SWCNT