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Open Dataset XSECPOP2

Spycatcher-Spytag-GFP complex

Abstract

SAXS of 1) Spycatcher003, 2) Spytag003-sfGFP, and 3) Spycatcher003 and Spytag003-sfGFP in complex

Experimental description

The X-ray wavelength was set at λ=1.127 Å and the sample to detector distance was 2100 mm, resulting in scattering vectors, q, ranging from 0.01 Å-1 to 0.47 Å-1. 60 μL of sample with a concentration ~3 mg/ml was prepared in the SEC running buffer. The Shodex KW803 column was equilibrated with a running buffer with a flow rate of 0.65 mL/min. Each sample was injected in an SEC column, and two-second X-ray exposures were recorded continuously for 24 min. BioXTAS RAW was used for further SEC-SAXS processing, including buffer subtractions and merging SAXS frames across the elution peak.

File description

1) Spycatcher003: S_A_Spycatcher.dat, 2) Spytag003-sfGFP: S_A_Spytag-GFP.dat, 3) Spytag003-sfGFP + Spycatcher003: A_S_Spycatcher_Spytag-GFP_complex.dat

Created

2025-04-14

Published

2025-04-16

Data collection technique

SEC-SAXS

Journal DOI

Source

Advanced Light Source

Beamline

SIBYLS BL12.3.1

Wavelength

1.24 Å

Sample to Detector Distance

2.083 m

Submitting Author

Joshua Del Mundo

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

[email protected]

Collaborators

Project Leader

Greg Hura

[email protected]


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Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.

Individual SAS Data Files (Total: 0)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.

Supplemental Data and Supporting Materials (Total: 0)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

Open SAXS data analysis sites in a new tab using these links

SAXS Similarity SAXS FrameSlice

Samples:

  • Macromolecule 1: Spycatcher
    • Sample Full Name: Spycatcher003
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System: Escherichia coli
    • Expression NCBI Taxonomy ID: 562
    • Uniprot ID's:
    • Sequence or Chemical Formula:
    • VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGRELAGATMELRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVD
  • Macromolecule 2: Spytag-GFP_Spycatcher_complex
    • Sample Full Name: Spytag003-sfGFP + Spycatcher003
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System: Escherichia coli
    • Expression NCBI Taxonomy ID: 562
    • Uniprot ID's:
    • Sequence or Chemical Formula:
    • VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGRELAGATMELRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVD: MGRGVPHIVMVDAYKRYKSGGGSGRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSGSGSHHHHHH
  • Macromolecule 3: Spytag-GFP
    • Sample Full Name: Spytag003-sfGFP
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System: Escherichia coli
    • Expression NCBI Taxonomy ID: 562
    • Uniprot ID's:
    • Sequence or Chemical Formula:
    • MGRGVPHIVMVDAYKRYKSGGGSGRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSGSGSHHHHHH