Open Dataset XSDJTRUW
SEC-SAXS-MALS for NSP7/NSP8 from SARS-CoV2
SEC-SAXS-MALS for NSP7/8 from SARS-CoV2
Experimental descriptionThe full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non Structural Proteins 7 and 8 are a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co- expressed in E. Coli and co-purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.
File descriptionEach file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP7/8.dat file for an SEC peak region is included.
2021-03-07
Published2021-05-21
Data collection techniqueSEC-SAXS
Journal DOI
Beamline
Wavelength
Sample to Detector Distance
Michal Hammel
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Collaborators
Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim
Project LeaderMichal Hammel
Data Download Terms:
The data available for download is free to use, however by clicking a download link, it signifies that you agree to our Terms of Service and Privacy Policy.
Complete Set of SAS Data Files
The complete SAS dataset is downloadable as a zip file.
- NSP7_8SEC-SAXSdata.zip Download
Individual SAS Data Files (total 1)
These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.
- Nsp7_8.dat Download
Supplemental Data and Supporting Materials (total 4)
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- 3ub0_dimercompact.pdb Download
- Nsp7_Nsp8 complex[050620_COVID_3].pdf Download
- nsp7_nsp8base_6yyt_6wiq.pdb Download
- NSP8_ensembles_size_1.txt.pdb Download
Open SAXS data analysis sites in a new tab using these links
SAXS Similarity SAXS FrameSlice