Open Dataset #XSDJTRUW
SEC-SAXS-MALS for NSP7/NSP8 from SARS-CoV2
Abstract: SEC-SAXS-MALS for NSP7/8 from SARS-CoV2
Experimental Description: The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non Structural Proteins 7 and 8 are a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co- expressed in E. Coli and co-purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.
Sample names and types:
- NSP7 | Non-structural Protein 7 | Protein
- NSP8 | Non-structural Protein 8 | Protein
File description: Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP7/8.dat file for an SEC peak region is included.
Data collection technique: SEC-SAXS
Submitted By: Michal Hammel | [email protected]
Project Leader: Michal Hammel | [email protected]
Additional Authors: Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim
Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline
Country: United States of America
Data Download Terms:
Complete Set of SAXS Data Files:
The complete SAXS dataset is downloadable as a zip file.
- NSP7_8SEC-SAXSdata.zip Download
Supplemental Data and Supporting Materials (total 4):
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- 3ub0_dimercompact.pdb Download
- Nsp7_Nsp8 complex[050620_COVID_3].pdf Download
- nsp7_nsp8base_6yyt_6wiq.pdb Download
- NSP8_ensembles_size_1.txt.pdb Download
Sample SAXS Data Files (total 1):
These are individual SAXS data files that are included in the complete dataset, but are available separately for quick download as examples of what is contained in the zip file.
- Nsp7_8.dat Download
Open SAXS data analysis sites in a new tab using these links:SAXS Similarity SAXS FrameSlice