Open Dataset XSDJTRUW
SEC-SAXS-MALS for NSP7/NSP8 from SARS-CoV2
SEC-SAXS-MALS for NSP7/8 from SARS-CoV2Experimental description
The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non Structural Proteins 7 and 8 are a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co- expressed in E. Coli and co-purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.File description
Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP7/8.dat file for an SEC peak region is included.
2021-05-21Data collection technique
Sample to Detector Distance
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang KimProject Leader
Data Download Terms:
Complete Set of SAS Data Files
The complete SAS dataset is downloadable as a zip file.
- NSP7_8SEC-SAXSdata.zip Download
Individual SAS Data Files (total 1)
These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.
- Nsp7_8.dat Download
Supplemental Data and Supporting Materials (total 4)
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- 3ub0_dimercompact.pdb Download
- Nsp7_Nsp8 complex[050620_COVID_3].pdf Download
- nsp7_nsp8base_6yyt_6wiq.pdb Download
- NSP8_ensembles_size_1.txt.pdb Download
Open SAXS data analysis sites in a new tab using these linksSAXS Similarity SAXS FrameSlice
- Macromolecule 1: NSP7
- Sample Full Name: Non-structural Protein 7
- Sample Type: Protein
- Source Organism:
- Source Organism NCBI Taxonomy ID:
- Expression System:
- Expression NCBI Taxonomy ID:
- Sequence or Chemical Formula:
- Macromolecule 2: NSP8