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Open Dataset XSDJTRUW

SEC-SAXS-MALS for NSP7/NSP8 from SARS-CoV2

Abstract

SEC-SAXS-MALS for NSP7/8 from SARS-CoV2

Experimental description

The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non Structural Proteins 7 and 8 are a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co- expressed in E. Coli and co-purified, sent to SIBYLS for collection and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

File description

Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP7/8.dat file for an SEC peak region is included.

Created

2021-03-07

Published

2021-05-21

Data collection technique

SEC-SAXS

Journal DOI

https://doi.org/10.1016/j.bpj.2021.06.006

Source

Advanced Light Source

Beamline

SIBYLS BL12.3.1

Wavelength

1.217 Å

Sample to Detector Distance

2.07 m

Submitting Author

M. Hammel

Lawrence Berkeley National Laboratory, The SIBYLS Beamline

United States of America

Collaborators

G. Hura, D. Rosenberg, M. Wilamowski, A. Joachimiak, Y. Kim

Project Leader

M. Hammel


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Complete Set of SAS Data Files

The complete SAS dataset is downloadable as a zip file.

Individual SAS Data Files (Total: 1)

These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.

Supplemental Data and Supporting Materials (Total: 4)

These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.

Open SAXS data analysis sites in a new tab using these links

SAXS Similarity SAXS FrameSlice

Samples:

  • Macromolecule 1: NSP7
    • Sample Full Name: Non-structural Protein 7
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Uniprot ID's:
    • Sequence or Chemical Formula:
  • Macromolecule 2: NSP8
    • Sample Full Name: Non-structural Protein 8
    • Sample Type: Protein
    • Source Organism:
    • Source Organism NCBI Taxonomy ID:
    • Expression System:
    • Expression NCBI Taxonomy ID:
    • Uniprot ID's:
    • Sequence or Chemical Formula: