Open Dataset XS7MRNJA
SEC-SAXS-MALS for NSP8 + dsRNA from SARS-CoV2
NSP8/dsRNA complexationExperimental description
The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non-Structural Proteins 8 is a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. These proteins were co-expressed in E. Coli and co- purified, sent to SIBYLS for collection. NSP8 was mixed with an equimolar concentration of dsRNA and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.File description
Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP78_dsRNAdat file for an SEC peak region is included.
2021-05-21Data collection technique
Sample to Detector Distance
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang KimProject Leader
Data Download Terms:
Complete Set of SAS Data Files
The complete SAS dataset is downloadable as a zip file.
- NSP8_dsRNA.zip Download
Individual SAS Data Files (total 1)
These are individual SAS data files that may be raw or processed (merged, etc.). These may or not be included in a zip file containing a larger dataset.
- nsp8rnapeak1.dat Download
Supplemental Data and Supporting Materials (total 2)
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
Open SAXS data analysis sites in a new tab using these linksSAXS Similarity SAXS FrameSlice
- Macromolecule 1: NSP8
- Sample Full Name: Non-structural Protein 8
- Sample Type: Protein
- Source Organism:
- Source Organism NCBI Taxonomy ID:
- Expression System:
- Expression NCBI Taxonomy ID:
- Sequence or Chemical Formula:
- Macromolecule 2: dsRNA