Open Dataset XSXMU3AK
SEC-SAXS-MALS for NSP8 + ssRNA from SARS-CoV2
NSP8 + ssRNA complexationExperimental description:
The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non-Structural Proteins 8 is a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. Theis proteins were expressed in E. Coli and purified, sent to SIBYLS for collection. NSP8 was mixed with an equimolar concentration of ssRNA and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.File description:
Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP8_ssRNAdat file for an SEC peak region is included.
Updated: 2021-05-21Submitting Author:
Lawrence Berkeley National Laboratory, The SIBYLS Beamline
United States of America
Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang KimProject Leader:
Data Download Terms:
Complete Set of SAXS Data Files:
The complete SAXS dataset is downloadable as a zip file.
- NSP8_ssRNA.zip Download
Sample SAXS Data Files (total 1):
These are individual SAXS data files that are included in the complete dataset, but are available separately for quick download as examples of what is contained in the zip file.
- nsp8_ssRNA.dat Download
Supplemental Data and Supporting Materials (total 1):
These data and materials may include X-ray crystal structure coordinates, multi-angle light scattering data, .etc. It may also include additional details or methods pertaining to the SAXS experiments, or the researcher's interpretations of the results.
- NSP8_ssRNA_MALS.pdf Download
Open SAXS data analysis sites in a new tab using these links:SAXS Similarity SAXS FrameSlice
- Macromolecule 1: NSP8
- Sample Full Name: Non-structural Protein 8
- Sample Type: Protein
- Source Organism:
- Source Organism NCBI Taxonomy ID:
- Expression System:
- Expression NCBI Taxonomy ID:
- Sequence or Chemical Formula:
- Macromolecule 2: ssRNA