Open Dataset #XSXMU3AK


Abstract: NSP8 + ssRNA complexation

Experimental Description: The full SEC-SAXS trace set is available here. Each fame is a 2 second exposure and since collected on a direct detector there is nearly no time separation between frames. The buffer was 20mM Hepes, 150 mM NaCl, 1 mM TCEP pH 7.5. Non-Structural Proteins 8 is a component of the RNA dependent RNA polymerase (RdRp) from SARS CoV2. Theis proteins were expressed in E. Coli and purified, sent to SIBYLS for collection. NSP8 was mixed with an equimolar concentration of ssRNA and then purified again as part of the SEC-SAXS process. The full SEC-SAXS elution set is here for further analysis.

Status: Published

Sample names and types:

  • NSP8 | Non-structural Protein 8 | Protein
  • ssRNA | Single-stranded RNA | RNA

File description: Each file name is numerically iterated so that *_00009.cbf.dat is the 9th SAXS frame from the elution trace. Since it is the 9th frame it is collected 18 seconds after the instrument began recording. The merged NSP8_ssRNAdat file for an SEC peak region is included.

Data collection technique: SEC-SAXS

Submitted By: Michal Hammel | [email protected]

Project Leader: Michal Hammel | [email protected]

Additional Authors: Greg Hura, Daniel Rosenberg, Mateusz Wilamowski, Andrzej Joachimiak , Youngchang Kim

Institute: Lawrence Berkeley National Laboratory, The SIBYLS Beamline

Country: United States of America

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Complete Set of SAXS Data Files:

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Supplemental Data and Supporting Materials (total 1):

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Sample SAXS Data Files (total 1):

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